Part:BBa_K137023:Design

GFP with (A)23 repeat after start codon

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 667
  

Design Notes

With 23 A repeats, the GFP sequence is in the -1 frame with respect to the start codon, so GFP should not be transcribed.

Source

The nucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run.